Figure 10. Effects of signal pathway inhibitors on the differentiation of NSCs in 4% HCM.

DMSO was used as the control condition. Both LY294002 and SP600125 suppressed the ability of NSCs to differentiate into neurons. The lowest proportions of β-TubIII+ neurons and the highest proportion of GFAP+ astrocytes were observed in LY294002. ^**p<0.01, SP600125 compared with DMSO; ^##p<0.01, LY294002 compared with DMSO. ^ΔΔp<0.01, LY294002 compared with SP600125 (n = 3).