Figure 5. Pharmacological pan-PIM kinase inhibition in PTCL cell lines.

(A) The pan-PIMi ETP-39010 reduced cell viability in all PTCL cell lines (IC₅₀ values calculated after 72 h of treatment are shown). (B) The pan-PIMi ETP-39010 strongly induced apoptosis in a time-dependent manner in all PTCL cell lines (*, p<0.05, from comparison with DMSO-treated cells). The percentage of non-viable cells was calculated as Annexin V+/PI− plus Annexin V+/PI+ cells in the PIMi-treated condition minus the DMSO-treated control. (C) The pan-PIMi ETP-39010 (10 µM for 48 h) slightly but significantly reduced cell viability only in tumoral T cells from 8 PTCL patients (Mycosis Fungoides and Sézary Syndrome), but did not affect normal T cells from 5 healthy donors (*, p<0.05 compared with DMSO).