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SR data for the relaxation enhancement due to Cu2+ binding for 23L/131R1 (A) and 23L/131R1p (B), in the absence (black) and presence (green) of saturated Cu2+. Fits to each trace using a single exponential function are shown in red, with corresponding residual signal provided below the SR data. The time constants from each fit and the Cu2+-nitroxide distance calculated based on the fast motional limit are reported in the inset. For 23L/131R1, no enhancement was observed.
