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. 2014 May 7;136(23):8450–8458. doi: 10.1021/ja503545r

Scheme 3. Final Assembly by NCL of Full-Length β-Subunit of hLH Bearing Chitobiose at the N-Glycosylation Site.

Scheme 3

NCL buffer: guanidine·HCl (6 M), Na2HPO4 (0.2 M), tris(2-carboxyethyl)phosphine hydrochloride (TCEP·HCl) (0.02 M), 4-mercaptophenylacetic acid (MPAA) (0.2 M), aqueous TCEP solution (0.5 M) pH 7.2–7.4.