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. 2014 May 17;136(24):8669–8676. doi: 10.1021/ja502372j

Figure 6.

Figure 6

Labeling of KATs in cell lysates. (a) Labeling of pCAF in HEK-293 overexpression extracts. Comp. = competitor. (b) Proteins identified in LC–MS/MS experiments as targets of H3K14-CoA-BPyne 2 in pCAF transfected HEK-293 extracts. Control = competitor treated lane, EV = extract derived from HEK-293 cells transfected with empty vector. (c) Limit of detection of recombinant pCAF spiked into HeLa cell proteome. Conditions: 2 (1 μM), proteome (7 μg), recombinant pCAF (0, 6.25, 5, 3.75, 2.5, 1.25 pmol).