Figure 5.
Replication fork progression is impaired in WRN-deficient and mutant cells. (A) Experimental scheme of dual labeling of DNA fibers. (B) Analysis of replication fork velocity in WSWRN (wild-type), WRN-deficient (WS) and mutant cells (WSWRN6A and WRN-K577M) under unperturbed conditions or (C) exposed to 0.4 μM Aph as indicated in (A). The length of the green tracks was measured. Median values are represented as horizontal black lines. (D) Representative images of actual DNA fibers from WSWRN, WRN-deficient and mutant cells. Scale bars, 10 μm. **** = Statistically significant P < 0.0001 (Student's t-test).