Figure 6.
Replication fork restart is affected by roscovitine in WRN-deficient and mutant cells. (A) Experimental scheme of dual labeling of DNA fibers in presence or absence of roscovitine. (B) Analysis of replication fork recovery in WSWRN (wild-type), WRN-deficient (WS) and mutant cells (WSWRN6A and WRN-K577M) treated as indicated in (A). Aph (0.4 μM) and roscovitine (100 μM) were added for the indicated time. Median values are represented as horizontal black lines. (C) Representative images of replication tracks from WSWRN, WRN-deficient and mutant cells with or without roscovitine treatment. (D) Percentage of new origin firing in cells treated as in (B). Data are reported as mean from three independent experiments. Error bars represent standard error. **** = Statistically significant P < 0.0001 (Student's t-test).