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. 2014 Oct 28;42(20):12555–12569. doi: 10.1093/nar/gku1033

Figure 2.

Figure 2.

Resveratrol reduces the stability of iNOS, IL-8 and TNF-α mRNA in human epithelial DLD-1 cells. DLD1 cells were treated with CM for 1 or 4 h. Afterwards, 30 μM resveratrol was added. Thirty minutes later the cells were treated with DRB (25 μg/ml) to stop RNA Polymerase II-dependent transcription. After 10, 30, 60 min, or 2, 4 and 6 h the expression of iNOS (A), IL-8 (B), TNF-α (C) and GAPDH (for normalization) mRNA was measured. iNOS, IL-8 or TNF-α mRNA expression was normalized to GAPDH mRNA expression. The relative iNOS, IL-8 and TNF-α mRNA expression after 1 or 4 h CM was set to 100%. Shown are the mean ± SEM of n = 10–12 analyses (###P < 0.001; ##P < 0.01; #P < 0.05; ns#, not significant versus cells not treated with resveratrol; two-way ANOVA). The half-lifes of the respective mRNAs are iNOS: CM 4.39 ± 0.54 h, CM + Res 2.86 ± 0.55 h; IL-8: CM 42.89 ± 11.11 min, CM + Res 20.16 ± 5.67 min; TNF-α: CM 25.80 ± 9.99 min, CM + Res 10.60 ± 4.75 min.