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. 2014 Oct 28;42(20):12555–12569. doi: 10.1093/nar/gku1033

Figure 3.

Figure 3.

Resveratrol reduces the stability of IL-8 and TNF-α mRNA in human monocytic Mono Mac 6 cells. (A and B) Mono Mac 6 cells were pre-incubated with 30 μM resveratrol (Res) for 1 h and then treated with CM for additional 2 h. RNA was isolated and analyzed for TNF-α (A), IL-8 (B) and GAPDH (for normalization) mRNA expression by qRT-PCR. Data shown are mean ± SEM of n = 8–10 qRT-PCR analyses. The CM-induced mRNA expression was set to 100% (***P < 0.001 versus CM-treated Mono Mac 6 cells; one-way ANOVA). (C and D) Mono Mac 6 cells were treated with CM for 2 h. Note that 30 μM resveratrol was added and 30 min later cells were treated with DRB (25 μg/ml) to stop RNA Polymerase II-dependent transcription. After 10, 30 and 60 min the expression of TNF-α (A), IL-8 (B) and GAPDH (for normalization) mRNA was measured. IL-8 or TNF-α mRNA expression was normalized to GAPDH mRNA expression. The relative IL-8 or TNF-α mRNA expression after 2 h CM was set to 100%. Shown are the mean ± SEM of n = 10–12 analyses (###P < 0.001; ##P < 0.01; #P < 0.05; ns#, not significant versus cells not treated with resveratrol; two-way ANOVA). The half-lifes of the respective mRNAs are IL-8: CM 20.39 ± 3.06 min, CM + Res 11.02 ± 1.60 min; TNF-α: CM 19.63 ± 6.10 min, CM + Res 7.87 ± 1.29 min.