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. 2014 Oct 28;42(20):12555–12569. doi: 10.1093/nar/gku1033

Figure 9.

Figure 9.

Resveratrol incubation enhances intracellular binding of KSRP to the IL-8, iNOS and TNF-α mRNA. DLD-1 cells were incubated for 2 (A and C) or 4 h (B) with CM with or without 30 μM resveratrol (Res). Cells were lyzed and RNA bound by KSRP protein was immunoprecipitated by a specific antibody. Immunoprecipitation with immunoglobulin G (IgG) was used as negative control. To normalize for subsequent RNA purification steps, 1 ng/sample in vitro transcribed luciferase RNA was added before the RNA was isolated from immunoprecipitated proteins. The amounts of IL-8, iNOS and TNF-α mRNA bound by KSRP were determined by qRT-PCR using the luciferase RNA as normalization control. (A) A summary of 14 immunoprecipitation-qRT-PCR analyses is shown. Columns (means ± SEM) represent relative IL-8 mRNA levels bound by KSRP (***P < 0.001 versus CM-treated DLD-1 cells; t-test). (B) A summary of 12 immunoprecipitation-qRT-PCR analyses is shown. Columns (means ± SEM) represent relative iNOS mRNA levels bound by KSRP (**P < 0.01 versus CM-treated DLD-1 cells, t-test). (C) A summary of 16 immunoprecipitation-qRT-PCR analyses is shown. Columns (means ± SEM) represent relative TNF-α mRNA levels bound by KSRP (***P < 0.001 versus CM-treated DLD-1 cells; t-test).