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. 2014 Oct 9;42(20):12735–12745. doi: 10.1093/nar/gku908

Figure 1.

Figure 1.

Tertiary (above) and secondary (below) structure representations of the yeast tRNAPhe constructs. The substrate position 55 (purple) was either a U55 (a) or a 5FU55 (b). The Cy5-label (red) was either attached at position U33 outside the minimal substrate (orange) (c) or at position C49 inside the minimal substrate (d). The enzyme TruB bound to the tRNA as predicted by a docking model (27) is shown in gray.