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. 2014 Oct 9;42(20):12735–12745. doi: 10.1093/nar/gku908

Figure 2.

Figure 2.

Analysis of SDS-stable complex formation of TruB with 5FU55- or U55-tRNA, respectively. Following 1 h incubation at 1 μM concentration and 70°C the complex was incubated with 0.5 vol SDS buffer for 5 min either at 25°C or at 95°C. (a) Coomassie stain for protein and (b) SYBR Gold stain for RNA. Note that SYBR Gold stains TruB faintly.