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. 2014 Oct 9;42(20):12735–12745. doi: 10.1093/nar/gku908

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Spectroscopic titration curves of TruB tRNA interaction (c_tRNA = 200 nM) for U55-tRNAs (a and b) and 5FU55-tRNAs (c and d), for all spectroscopic parameters with fits to a 1:1 binding model as solid lines. (e) Schematic representation of the chase experiment. (f) Chase experiments for C49-tRNA TruB interaction: The fraction bound is averaged over the values obtained from all spectroscopic parameters without TruB (free), with TruB (>90% binding) and after addition of unlabeled U55-tRNA in excess to the complex.