Figure 8.

Dominant-negative Prp28 mutations. PRP28 cells were transformed with 2μ HIS3 vector or 2μ HIS3 plasmids bearing the indicated PRP28 alleles under the transcriptional control of the GAL1 promoter. Liquid cultures derived from single transformants were grown to mid-log phase at 30°C in SD-His glucose medium and adjusted to the same A₆₀₀. Aliquots (3 μl) of serial 10-fold dilutions of cells in water were spotted to SD-His agar with 2% glucose or 2% galactose as indicated. The plates were photographed after incubation at 30°C for 2 days (glucose) or 4 days (galactose).