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. 2014 Oct 16;42(20):12415–12424. doi: 10.1093/nar/gku933

Figure 2.

Figure 2.

The role of DNA geometry in the distribution of the length of the sliding events at salt concentrations of (A) 0.04 M and (B) 0.10 M on 100 bp linear (black) and circular (red) DNA. The length of each sliding event was measured in terms of the total number of DNA base pairs probed via an uninterrupted sliding motion by the N-domain of Pax6 either when it was tethered to the C-domain (Pax6; solid lines) or as an isolated domain (Pax6N; dotted lines). ‘Uninterrupted’ means sliding that occurred without intervening hopping or 3D diffusion events. Where the protein revisited a previously probed DNA site, it was counted only once.