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. 2014 Oct 10;42(20):12768–12778. doi: 10.1093/nar/gku941

Figure 1.

Figure 1.

Ψ and 2′-O-methyl modifications increase the structural stability of (CUG)4 RNA. (A) Uridine and Pseudouridine. Pseudouridine (Ψ) is an isomerization product of uridine that has a C–C bond connecting the base to the ribose as well as an extra N–H bond donor. (B) Depictions of the structures of unmodified (CUG)4 RNA along with (CUG)4 with one, two and four Ψ and 2′-O-methyl modifications. (C) Melting curves for (CUG)4 RNAs modified with Ψ. The total change in melting temperature between unmodified (CUG)4 and 4Ψ(CUG)4 is ∼20°C (59±1, 67±2, 72±1 and 78±1°C for (CUG)4, 1Ψ(CUG)4, 2Ψ(CUG)4 and 4Ψ(CUG)4). (D) Melting curves for (CUG)4 RNAs modified with 2′O methyl groups. The total change in melting temperature between unmodified (CUG)4 and 4–2′Ome(CUG)4 is ∼7°C (62±2, 62±1 and 66±1°C for 1–2′Ome(CUG)4, 2–2′Ome(CUG)4 and 4–2′Ome(CUG)4).