Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Oct 10;42(20):12768–12778. doi: 10.1093/nar/gku941

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Binding gels and curves for MBNL1-(CUG)₄ RNA interactions. (A) Binding gels from MBNL1-(CUG)₄ affinity assays for RNAs modified with Ψ. RNAs were radiolabeled with α-P³² and combined with different concentrations of a consensus MBNL1 sequence (amino acids 2–260). RNA–protein complex formation induces a band-shift due to an increase in size. K_Ds were calculated based on the signal from the radiolabeled RNA and values are approximately the [MBNL1] when 50% of the RNA is bound. (B) Binding gels from MBNL1-(CUG)₄ affinity assays for RNAs modified with 2-’O-methylation. (C) Binding curves for (CUG)₄ RNAs modified with Ψ. Data were fit to the following equation to determine K_D values: f_bound = f_max([MBNL1]/([MBNL1 + K_D)). (D) Binding curves for (CUG)₄ RNAs modified with 2′-O-methylation. K_D values were calculated using the above equation.