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. 2014 Oct 28;42(20):12789–12805. doi: 10.1093/nar/gku952

Figure 4.

Figure 4.

The effect of vsRNA1 on virus infection and viral protein synthesis. (A) Effect of anti-vsRNA1 sponge RNA on EV71 viral growth. EV71-infected RD cells were transfected with the anti-vsRNA1 sponge or control RNA. After viral adsorption, viruses from the debris and the supernatant were collected at 4, 8 and 12 h p.i. The viral yields were determined with plaque assays. Error bars represent standard deviations for duplicate assays (*P < 0.05; **P < 0.01, Student's t-test). (B) Effect of anti-vsRNA1 sponge RNA on EV71 replication. The luciferase activity in the EV71 replicon in RD cells cotransfected with anti-vsRNA1 sponge or control RNA was monitored at 4, 6, 9, 12 and 15 h post-transfection. Error bars represent standard deviations for three independent experiments (*P < 0.01; ***P < 0.001; Student's t-test). (C) Effects of anti-vsRNA1 sponge on viral and host protein synthesis. Mock- or EV71-infected cells were transfected with the anti-vsRNA1 sponge or control RNA. Protein synthesis in these cells was examined using 35S-methionine/cysteine-labelling between 6 and 7 h p.i. The labelled viral proteins were identified according to their sizes. Viral protein 3C in the cell lysates was detected by western blotting. (D) Effect of vsRNA1 on EV71 viral growth. EV71-infected RD cells were transfected with vsRNA1 mimic or scrambled RNA. After viral adsorption, viruses from the debris and the supernatant were collected at 4, 8 and 12 h p.i. The viral yields were determined using plaque assays. Error bars represent standard deviations for duplicate assays (***P <0.001), Student's t-test). (E) Effect of the vsRNA1 mimic on EV71 replication. Luciferase activity in the EV71 replicon in RD cells cotransfected with vsRNA1 mimic or scrambled RNA was monitored at 4, 6, 9, 12 and 15 h post-transfection. Error bars represent standard deviations for three independent experiments (*P < 0.05, ***P < 0.001, Student's t-test). (F) Effects of vsRNA1 on viral and host protein synthesis. Mock- or EV71-infected cells were transfected with vsRNA1 mimic or scrambled RNA (Scramb). Protein synthesis in these cells was examined using 35S-methionine/cysteine-labelling between 4 and 5 h p.i. The labelled viral proteins were identified according to their sizes. Viral protein 3C in the cell lysates was detected by western blotting.