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. 2014 Oct 16;42(20):12483–12497. doi: 10.1093/nar/gku953

Figure 3.

Figure 3.

Phosphorylation of Ser4/6 on hnRNPA1 regulates BCL-XL and XIAP expression. (A and B) HEK293 cells were transiently transfected with wild-type (WT), mutant (S4AS6A or S4DS6D) hnRNPA1 or vector alone (V). (A) Cells were treated ± FGF-2 for 4 h prior to SDS-PAGE/western blotting for the indicated proteins. Lower panel: the BCL-XL and XIAP signals were quantified and normalized to that of HSP90. (B) Cells were subsequently transfected with reporter mRNAs driving CAT from the BCL-XL or XIAP IRES. CAT activity was normalised to the amount of injected mRNA. CAT alone mRNAs were used as control. Results are average of (A, lower panel) three independent experiments ± SEM or (B) triplicates ± SEM. Student's t-test: *P < 0.05; **P < 0.01.