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. 2014 Oct 16;42(20):12483–12497. doi: 10.1093/nar/gku953

Figure 4.

Figure 4.

hnRNPA1 shuttles between cytoplasm and nucleus in response to FGF-2. HEK293 cells alone (A), pre-treated with the MEK inhibitors PD098059, U1026 or DMSO alone for 1 h (B), transfected with siRNA for S6K2 (C), TNPO1 and 2 (E) or NXF1 (G) or the karyopherin inhibitor MycMBP-M9M vector or vector alone (D) were treated ± FGF-2 for the indicated time. The cytoplasmic fraction was analysed by SDS-PAGE/western blotting (WB) for the indicated proteins. S6K2 (C), TNPO1 and 2 (E) or NXF1 (G) knockdown were confirmed by SDS-PAGE/WB (C and G) or qPCR (E, lower panel). (A–E and G Lower panel) The hnRNPA1 signal was quantified and normalized to that of tubulin. (F) Endogenous hnRNPA1 was immunoprecipitated from HEK293 cells treated ± FGF-2. Immunoprecipitates were analysed by SDS-PAGE/WB for the indicated proteins. (A–G) Results are representative of at least three independent experiments. Bar graphs are average from replicate experiments ± SEM. See also Supplementary Figure S2.