. 2014 Oct 27;42(20):12912–12927. doi: 10.1093/nar/gku960

Table 1. Elution volumes, experimental and theoretical molecular weights of RPA, Tim-Tipin and Tim-Tipin-RPA.

Protein complex	Elution volume [ml]	Experimental^SEC# molecular weight [kDa]	Experimental^SLS& molecular weight [kDa]	Experimental^SG* molecular weight [kDa]	Theoretical^§ molecular weight [kDa]
RPA	1.63 ± 0.02	110	106 ± 6	N.D.	96.2
Tim-Tipin	1.46 ± 0.01	321	164 ± 12	158	162.3
Tim-Tipin-RPA	1.43 ± 0.02	388	273 ± 31	N.D.	258.5

^#Molecular weights derived from SEC were estimated based on the comparison with molecular mass standard proteins.

^&Molecular weights determined by SLS.

*Molecular weight determined by sucrose-gradient sedimentation assay (SG).

^§Molecular weights calculated based on the amino acid sequence.

N.D. = not determined.