Table 2. Elution volumes, 260/280 ratio, experimental and theoretical molecular weights of RPA, Tim-Tipin (TTP) and Tim-Tipin-RPA in presence of ssDNA substrates.

DNA substrate/ Protein:ssDNA [μM]	Protein mixture/ DNA substrate	Elution volume* [ml]	260/280*	ExperimentalSEC# molecular weight [kDa]	Theoretical§ molecular weight [kDa]
	RPA	1.63 ± 0.02	0.63 ± 0.02	110	96.2
	Tim-Tipin	1.46 ± 0.01	0.55 ± 0.01	321	162.3
	Tim-Tipin-RPA	1.43 ± 0.02	0.56 ± 0.01	388	258.5
60 nt	RPA	1.54 ± 0.00	1.57 ± 0.05	194	114.6
16:32	Tim-Tipin	1.44 ± 0.01	0.98 ± 0.14	364	180.7
	Tim-Tipin-RPA	1.39 ± 0.01	1.20 ± 0.04	498	276.9
60 nt	RPA	1.38 ± 0.01	1.32 ± 0.03	531	403.2
32:8	Tim-Tipin	1.47 ± 0.01	0.74 ± 0.06	301	180.7
	Tim-Tipin-RPA	1.44 ± 0.01 (TTP)	0.66 ± 0.05	364
		1.33 ± 0.05 (RPA)	1.13 ± 0.04	726
31 nt	RPA	1.55 ± 0.03	1.46 ± 0.01	182	105.7
16:32	Tim-Tipin	1.46 ± 0.02	0.74 ± 0.01	321	171.8
	Tim-Tipin-RPA	1.40 ± 0.03	1.11 ± 0.04	468	268.0
31 nt	RPA	1.48 ± 0.02	1.28 ± 0.05	283	201.9
16:8	Tim-Tipin	1.47 ± 0.01	0.57 ± 0.02	301	171.8
	Tim-Tipin-RPA	1.41 ± 0.03 (TTP)	0.87 ± 0.0	439
		1.38 ± 0.03 (RPA)	0.98 ± 0.01	531
14 nt	RPA	1.63 ± 0.0	1.47 ± 0.24	110	100.5
16:32	Tim-Tipin	1.48 ± 0.01	0.54 ± 0.02	283	166.6
	Tim-Tipin-RPA	1.45 ± 0.02 (TTP)	0.66 ± 0.08	342
		1.62 ± 0.01 (RPA)	1.27 ± 0.05	117
14 nt	RPA	1.63 ± 0.0	1.27 ± 0.06	110	100.5
16:8	Tim-Tipin	1.48 ± 0.01	0.54 ± 0.0	283	166.6
	Tim-Tipin-RPA	1.45 ± 0.02 (TTP)	0.64 ± 0.05	342
		1.62 ± 0.01 (RPA)	1.22 ± 0.04	117
ssDNA only	60 nt	1.70	1.82	64	18.4
0:16	31 nt	1.70	1.76	64	9.5
	14 nt	1.88	2.34	21	4.3
	Thyroglobulin	1.32 ± 0.02*			670
	γ-globulin	1.63 ± 0.03*			158
	Ovalbumin	1.76 ± 0.01*			44
	Myoglobin	1.91 ± 0.02*			17
	Vitamin B12	2.15 ± 0.02*			1.35

^#Molecular weights estimated based on the comparison with molecular mass standard proteins.

^§Molecular weights calculated based on the amino acid sequence.

* Average numbers and standard deviations of at least two independent measurements are shown.