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. 2014 Oct 21;42(20):12614–12627. doi: 10.1093/nar/gku993

Figure 2.

Figure 2.

Purified fragments of RecQL4. (A) Schematic depiction of the full-length protein and the fragments including the tags used for purification. The C-terminal fragment contained the helicase domain. (B) Coomassie blue-stained SDS-PAGE gel of purified recombinant N- and C-terminal fragments expressed in E. coli and the full-length protein expressed in baculovirus-infected High-Five™ insect cells. Tags (FLAG, 6HIS, GST), the homeo-like N-terminal 54 residues homologous to the yeast Sld2 protein and the helicase domain are indicated.