Figure 5.

The N-terminus of RecQL4 favored binding to Y-shaped DNA. (A) EMSAs were performed with 1.3 μM of ss-, ds- or Y-shaped LB2-derived DNA and the indicated amounts of RecQL4_N170. (B) Quantification of EMSA assays of the N-terminal fragments and 1.3 μM of ss-, ds- or Y-shaped LB2-derived DNA. (C) Comparison of DNA binding of RecQL4_N388 and RecQL4_FL using the same substrates as in (A) and (B). All DNA-binding experiments and quantifications presented here were performed under identical conditions. All experiments were performed up to four times with minor variations in the concentration of the DNA substrates and produced in all cases comparable results.