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. 2014 Oct 21;42(20):12707–12721. doi: 10.1093/nar/gku997

Figure 3.

Figure 3.

6Ala TH stabilization detected by CPR mapping of TL/TH conformation. (A) Schematic of F937–736 (orange) and U937–1139 (green) CPRs detecting the folded TH and unfolded TL conformations. (B) F937–736 and 6Ala F937–736 fraction crosslinked (orange) and U937–1139 and 6Ala U937–1139 (green; contains 5 of the 6Ala residues in the 6Ala RNAP, sixth residue is Cys in this variant). Bar graphs show the fraction crosslinked at saturating levels (EH –0.136 V) for free core RNAP or EC 3′deoxyG13 (ScaffoldGTP; Figure 2A; EC G13 containing 3′-dGMP) in the presence and absence of 10 mM GTP. Data are means ± SD of experimental triplicates.