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. 2014 Oct 21;42(20):12707–12721. doi: 10.1093/nar/gku997

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 7. — SI3 effects on elongation, pausing, and CPR mapping of TL/TH conformation. (A) Single-round NTP addition rates with Scaffold^UTP (Fgure 4) for wild-type and ΔSI3 RNAPs under predicted UTP saturation levels (10 mM). ΔSI3a corresponds to Δ1043–1130 and ΔSI3b corresponds to Δ1045–1132. (B) Comparison of his pause kinetics on scaffold^PEC (as in Figure 5) for wild-type, ΔSI3a and ΔSI3b RNAPs. (C) Comparison of UTP addition kinetics (as in Figure 4) for RNAP variants ± ΔSI3. (D) F937–736 ± ΔSI3 and 6Ala F937–736 ± ΔSI3 fraction crosslinked as a function of redox potential (2.5 mM CSSC, varying DTT) in free core RNAP, EC (Scaffold^GTP) and PEC (Scaffold^PEC at pause). Data are means ± SD of experimental triplicates.