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A triple mutant box showing the effects, in kcal/mol, of single amino acid mutations on (ΔG⧧)OMP for reactions catalyzed by wild-type OMPDC (black values), by single mutants of OMPDC (red values), and by double mutants of OMPDC (green values). These effects are reported as (ΔΔGX∓)OMP for the mutations, calculated from the ratio of the kinetic parameters kcat/Km for precursor and mutated OMPDC-catalyzed decarboxylation of OMP, which are reported in Table 3.
