Fig. 2.

Time course (A) of total sperm motility and effects of SAC at 75 weeks (B). A portion of the sperm suspension was diluted with TYH medium and incubated at 37°C for 5 min in a CO₂ incubator. A 10-µl aliquot of the sperm solution (1:100) was then immediately transferred to a slide chamber for a computer-aided sperm motility analysis on a thermo plate under an optical microscope. Total sperm motility (A) was calculated as the percentage of motile sperm in the total sperm concentration. Values are the mean ± SEM as a percentage of the motility at 25 weeks of age, which was set equivalent to 100% (n = 4–5). *p<0.05, **p<0.01 compared to 25 weeks of age. (B) Total sperm motility at 75 weeks. ^#p<0.05.