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HPLC-MS traces of the (a) mixture of nucleosides used as a standard and (b) digestion results of transcript 5. Digestion of 1–2 nmol of transcript was carried out using S1 nuclease for 2 h at 37 °C and followed by dephosphorylation with alkaline phosphatase for 2 h at 37 °C. The ribonucleoside mixture obtained was analyzed by reverse-phase analytical HPLC, using a mobile phase of 0–6% acetonitrile (0.1% formic acid) in water (0.1% formic acid) over 12 min; flow rate 1 mL/min.
