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. 2014 Nov 11;9(11):e112115. doi: 10.1371/journal.pone.0112115

Figure 5. AGE-modification of PC12 cells using MGO.

Figure 5

PC12 cells were incubated with PBS, 0.1 mM MGO, 0.3 mM MGO or 1.0 mM MGO for 4 hours. A. Washed cells were solubilized and subjected to SDS-gel electrophoresis. Proteins were blotted and detected using monoclonal CML26 antibody B&C. Permeabilized (B) and non-permeabilized (C) were analyzed by flow cytometry using monoclonal CML26 antibody.