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. 2014 Nov 11;9(11):e112699. doi: 10.1371/journal.pone.0112699

Figure 3. Inhibition of Sirt1 in HUVECs induced premature senescence.

Figure 3

(A) and (B) Profiles of Sirt1 mRNA expression and Sirt1 protein expression 24 hours after transfection with Sirt1 siRNA. (C) and (D) Profiles of PAI-1 mRNA expression and PAI-1 protein expression 72 hours after transfection with Sirt1 siRNA. (E) SA-β-gal staining was done 72 hours after Sirt1 siRNA transfection. *P<0.05 vs Mock group, n = 3. Mock, transfection reagent only group; NC, negative control siRNA group; siRNA, Sirt1 siRNA group.