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. 2014 Nov 11;9(11):e112699. doi: 10.1371/journal.pone.0112699

Figure 4. Rb1 does not inhibit Sirt1 depletion-induced senescence.

Figure 4

(A) The Sirt1 protein level was evaluated by a Western blot analysis 72 hours after siRNA transfection with or without Rb1 supplementation. (B) The PAI-1 protein expression was examined 72 hours after siRNA transfection. (C) A total of 400 cells were counted in order to calculate the ratio of SA-β-gal–positive cells 72 hours after siRNA transfection. *P<0.01 vs Mock; NS, P>0.05 vs siRNA; n = 3. Mock, transfection reagent only group; Rb1, 20 µmol/L Rb1-treated group; siRNA, Sirt1 siRNA group; siRNA+Rb1, Sirt1 siRNA plus 20 µmol/L Rb1-treated group.