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. 2014 Nov 11;9(11):e112886. doi: 10.1371/journal.pone.0112886

Figure 6. Refined models of the nidogen-1 G3/laminin γ1 LEb2–4 complex.

Figure 6

Based on PDB entry 1NPE and the identified cross-links, modified structural models for the high-affinity interaction region of laminin γ1 and nidogen-1 were generated. Cross-linked residues are displayed as spheres. Cα–Cα distances are given in Å. (A) Model with the best Rosetta total score and a Rosetta atom-pair constraint score ranking among the top 2.5%. (B) Model with the best Rosetta atom-pair constraint score and a Rosetta total score ranking among the top 2.5%. (C) Alignment of both models and the unmodified crystal structure 1NPE (black). The orientation of LEb2–4 clearly has changed during structural refinement. The β-propeller fold of the G3 domain is still intact.