Table 3.
Top ten up- and down-regulated NAc transcripts between HVRrun and LVRrun but similar between HVRnon-run and LVRnon-run rats (scenario in Fig.1A)
| HVRrun | LVRrun | HVRrun/ | HVRnon-run | LVRnon-run | HVRnon-run/ | |||
|---|---|---|---|---|---|---|---|---|
| Transcript | RPKM avg | RPKM avg | LVRrun | P | RPKM avg | RPKM avg | LVRnon-run | P |
| Up-regulated in HVRrun and LVRrun but were similar between HVRnon-run and LVRnon-run | ||||||||
| Follistatin (Fst), mRNA | 2.30↑ vs. HVRnr | 1.37 | 1.68 | 0.002 | 1.41 | 1.59 | −1.13 | 0.449 |
| Serum/glucocorticoid regulated kinase 1 (Sgk1), mRNA | 41.20 | 26.97 | 1.53 | 0.009 | 31.52↑ vs. CTL | 27.59↑ vs. CTL | 1.14 | 0.380 |
| Dymeclin (Dym), mRNA | 2.46↑ vs. HVRnr | 1.67 | 1.47 | 0.000 | 1.81 | 1.89 | −1.04 | 0.678 |
| Protocadherin gamma subfamily A8 (Pcdhga8), mRNA | 3.71↑ vs. HVRnr | 2.56 | 1.45 | 0.002 | 2.76 | 2.64 | 1.05 | 0.520 |
| Protocadherin gamma subfamily A11 (Pcdhga11), mRNA | 2.39↑ vs. HVRnr | 1.65 | 1.44 | 0.000 | 1.89 | 1.83 | 1.04 | 0.693 |
| HAUS augmin-like complex, subunit 4 (Haus4), mRNA | 4.58 | 3.17 | 1.44 | 0.002 | 4.83 | 3.62 | 1.33 | 0.062 |
| Zinc finger protein 189 (Zfp189), mRNA | 11.66↑ vs. HVRnr | 8.34 | 1.40 | 0.009 | 8.03↑ vs. CTL | 7.95↑ vs. CTL | 1.01 | 0.938 |
| Solute carrier family 38, member 10 (Slc38a10), mRNA | 1.68 | 1.21 | 1.38 | 0.004 | 1.41 | 1.38 | 1.02 | 0.818 |
| Leucine rich repeat containing 8 family, member C (Lrrc8c), mRNA | 6.09↑ vs. HVRnr | 4.47 | 1.36 | 0.004 | 4.80 | 4.62 | 1.04 | 0.778 |
| Transmembrane protein 38A (Tmem38a), mRNA | 2.21↑ vs. HVRnr | 1.63 | 1.36 | 0.006 | 1.71 | 1.46 | 1.17 | 0.073 |
| Down-regulated in HVRrun and LVRrun but similar in HVRnon-run and LVRnon-run | ||||||||
| Steroid receptor RNA activator 1 (Sra1), non-coding RNA | 1.93↓ vs. HVRnr | 2.67 | −1.39 | 0.001 | 2.60 | 2.73 | −1.05 | 0.706 |
| Bcl2 modifying factor (Bmf), mRNA | 3.02↓ vs. HVRnr | 4.21 | −1.39 | 0.002 | 4.44 | 4.14 | 1.07 | 0.538 |
| Zinc finger protein 408 (Znf408), mRNA | 1.56↓ vs. HVRnr | 2.22 | −1.42 | 0.001 | 2.16 | 2.04 | 1.06 | 0.357 |
| PREDICTED: Rattus norvegicus zinc finger CCCH type, antiviral 1-like (LOC100365858), miscRNA | 1.23↓ vs. HVRnr | 1.77 | −1.43 | 0.003 | 1.66 | 1.73 | −1.04 | 0.748 |
| PREDICTED: Rattus norvegicus SRY-box containing gene 9 (LOC100361122), mRNA | 2.53 | 3.66 | −1.45 | 0.007 | 3.31 | 3.40 | −1.03 | 0.844 |
| Patatin-like phospholipase domain containing 7 (Pnpla7), mRNA | 1.49↓ vs. HVRnr | 2.17 | −1.45 | 0.004 | 2.05 | 1.94 | 1.05 | 0.327 |
| ADAM metallopeptidase with thrombospondin type 1 motif, 4 (Adamts4), mRNA | 12.38 | 18.08 | −1.46 | 0.005 | 15.08 | 16.99 | −1.13 | 0.317 |
| RTEL1-TNFRSF6B readthrough, non-coding RNA | 2.07 | 3.83 | −1.85 | 0.006 | 2.92 | 3.43 | −1.17 | 0.311 |
| 3-Hydroxy-3-methylglutaryl-CoA synthase 2 (mitochondrial) (Hmgcs2), mRNA | 3.15↓ vs. HVRnr | 5.83 | −1.85 | 0.002 | 4.57↓ vs. CTL | 5.34 | −1.17 | 0.204 |
| Keratin 2 (Krt2), mRNA | 1.10 | 2.05 | −1.87 | 0.002 | 1.29 | 1.77 | −1.37 | 0.093 |
These transcripts were the top 10 up- and down-regulated NAc transcripts from the 94 transcripts that were differentially expressed in HVRrun versus LVRrun rats, probably a result of more running in HVRrun rats. Abbreviations: HVRrun = HVR 34-day-old 6-day runners, LVRrun = LVR 34-day-old 6-day runners, HVRnon-run = HVR 34-day-old non-runners, LVRnon-run = LVR 34-day-old non-runners, CTL = control Wistar rats. Symbols: ↑ vs. CTL = transcript in HVRnon-run or LVRnon-run rats was higher than in CTL rats (P < 0.05); ↓ vs. CTL = transcript in HVRnon-run or LVRnon-run rats was lower than in CTL rats (P < 0.05); ↑ vs. HVRnon-run or LVRnon-run = transcript in HVRrun or LVRrun is greater than HVRnon-run or LVRnon-run rats, respectively (P < 0.05); ↓ vs. HVRnon-run or LVRnon-run = transcript in HVRrun is less than HVRnon-run rats (P < 0.05). These significant differences are in bold type.