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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: JAMA Neurol. 2014 Nov 1;71(11):1413–1420. doi: 10.1001/jamaneurol.2014.1432

Figure 3. Normal Localization of NEFL at Motor End Plates in Affected Muscle.

Figure 3

A and B, Indirect immunofluorescence shows similar localization of NEFL protein in transverse sections of control (A) and patient 20-2 (B) skeletal muscle biopsy specimens. C-E, Shown is double-label indirect immunofluorescence of a longitudinal section of myofiber from patient 20-2 stained for NEFL (green in C) and a-bungarotoxin (red in D). The merged image in E identifies NEFL-positive structures as motor end plates (scale bars are 50μm).