. Author manuscript; available in PMC: 2015 Nov 1.

Published in final edited form as: Mol Microbiol. 2014 Oct 20;94(4):926–944. doi: 10.1111/mmi.12808

Table 1.

F. tularensis LVS DsbA substrates identified by mass spectrometry

Locus^a	#Cys^b	Protein name^c	Accession	Localization^d	Size (kDa)	Reference
38	4	HlyD family secretion protein	gi\|89255485	IM	35
93	10	Chitinase	gi\|89255533	Peri	80
125	5	Hypothetical protein	gi\|89255562	Unk	120
174	6	Hypothetical protein	gi\|89255608	Unk	17
181	4	Type IV pili protein	gi\|89255615	Unk	15
187	2	Cyclohexadienyl dehydratase	gi\|89255621	Peri	27
207	2	Pyrrolidone-carboxylate peptidase	gi\|89255641	Peri	24
325	4	OmpA family protein	gi\|89255751	OM/Lipo	46	Huntley et al., 2007; Mahawar et al., 2012; Robertson et al., 2014
336	7	Pal	gi\|89255762	OM/Lipo	23	Huntley et al., 2007
359	2	Type IV pili protein	gi\|89255780	Unk	33
411	6	Hypothetical protein	gi\|89255822	Unk	32
421	3	Tul4-A	gi\|89255832	OM/Lipo	16	Huntley et al., 2007
424	3	Tul4-B	gi\|89255834	OM/Lipo	16	Huntley et al., 2007
466	3	Murein transglycosylase	gi\|89255876	Peri	72
493	20	Hypothetical protein	gi\|89255903	Peri	30
574	2	Hypothetical protein	gi\|89255972	OM	51
701	11	FAD binding family protein	gi\|89256090	Peri	65
823	3	Hypothetical protein	gi\|89256186	Unk	14
834	4	Rhodanese-like protein	gi\|89256194	Unk	27
878	3	DNA/RNA endonuclease family	gi\|89256236	Extracell	39
879	2	Beta-lactamase	gi\|89256237	Peri	32
994	7	Hypothetical protein	gi\|89256338	Unk	55
1029	4	Type IV pili lipoprotein	gi\|89256368	Unk	33
1042	4	MipA	gi\|89256380	OM/Lipo	30	Huntley et al., 2007; Rasko et al., 2008
1060	3	D-alanyl-D-alanine carboxypeptidase	gi\|89256395	IM	49
1064	4	Hypothetical protein	gi\|89256399	Unk	30
1105	2	Hypothetical protein	gi\|89256430	Unk	16
1161	4	PdpE/Hcp	gi\|89256483	Unk	21	Barker et al., 2009
1171	5	PdpB/IcmF	gi\|89256493	Unk	120	de Bruin et al., 2011
1202	9	Hypothetical protein	gi\|89256523	Unk	36
1286	3	Hypothetical protein	gi\|89256595	Unk	15
1306	4	DipA	gi\|89256615	OM	39	Chong et al., 2013
1328	2	FopA	gi\|89256632	OM	43	Huntley et al., 2007; Chong et al., 2013
1363	1	Hypothetical protein	gi\|89256662	Unk	37
1372	5	Hypothetical lipoprotein	gi\|89256671	OM/Lipo	49
1521	7	Chitinase family 18 protein	gi\|89256806	Unk	84
1532	3	Hypothetical protein	gi\|89256816	Unk	21
1548	4	Hypothetical protein	gi\|89256832	Unk	29
1570	5	Phospholipase D	gi\|89256847	Unk	45
1578	2	Hypothetical protein	gi\|89256855	Unk	13
1579	4	Hypothetical protein	gi\|89256856	Unk	26
1581	8	Hypothetical lipoprotein	gi\|89256858	OM/Lipo	30
1582	4	Putrescine-binding protein	gi\|89256859	Peri	43
1629	4	Hypothetical protein	gi\|89256906	Unk	62
1670	8	DsbB	gi\|89256943	Unk	18
1678	3	Hypothetical protein	gi\|89256948	Unk	37
1695	1	Hypothetical protein	gi\|89256964	Unk	29
1709	4	Hypothetical protein	gi\|89256978	Unk	18
1786	9	Succinate dehydrogenase	gi\|89257049	IM	66
1793	11	Hypothetical protein	gi\|89257056	Unk	104
1852	2	Hypothetical protein	gi\|89257112	Unk	15
1896	5	Hypothetical protein	gi\|89257144	Unk	51
1960	8	Peptide methionine sulfoxide reductase	gi\|89257196	Unk	25

F. tularensis LVS gene loci;

Number of cysteines in the predicted coding sequence;

Protein names in genome annotations or previous publications;

Localization predicted by PSORTb version 3.0.2 bacterial subcellular localization prediction program (http://www.psort.org), indicating inner membrane (IM), periplasmic (Peri), outer membrane (OM), or unknown (Unk) localization; lipoproteins (Lipo) predicted by LipoP 1.0 Server (http://www.cbs.dtu.dk/services/LipoP/); confirmed OM proteins are indicated in bold (OM) with relevant references.