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. Author manuscript; available in PMC: 2015 Jun 1.
Published in final edited form as: J Invest Dermatol. 2014 Jul 19;134(12):2908–2917. doi: 10.1038/jid.2014.263

Figure 6. PGP peptides from BP biological fluids affect PMN chemotaxis and PMN activation.

Figure 6

(a) PGP sequences (in red) within the C-terminal part of collagen XVII protein sequence. (b) PGP peptides detection by electrospray ionization liquid chromatography-MS/MS in serum from patients without inflammatory and/or autoimmune diseases, BP serum and BF. Data are shown as a box-and-whisker plot. The median of the distribution is indicated by the horizontal line in the box. Mann-Whitney test was used for statistics. (c) Chemotactic migration of PMN induced by PGP, PGPPGP or AcPGP peptides (10 µM) in a Boyden chamber chemotaxis assay (n=3). (d) HNE activities in PGP, PGPPGP or AcPGP-stimulated PMNs relative to control (n=3). The error bars denote the mean± SEM. Student t-test was used for statistics.