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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Mol Microbiol. 2014 Oct 16;94(4):898–912. doi: 10.1111/mmi.12807

Figure 2.

Figure 2

CTA1 structure in the absence or presence of ARF6.

Curve fitting (left panels) and second derivatives (right panels) for the FTIR spectrum of 13C-lableled CTA1 are shown. For curve fitting, the dotted line represents the sum of all deconvoluted components (solid lines) from the measured spectrum (dashed line). All spectra were recorded at 37°C with the exception of the 10°C spectrum presented in panel A.

A. CTA1 was incubated at 10°C for 30 min.

B. CTA1 was incubated at 37°C for 30 min.

C. CTA1 was mixed with ARF6/GTP at 4°C before warming to 37°C for 30 min.

D. CTA1 was mixed with ARF6/GDP at 4°C before warming to 37°C for 30 min.

E. CTA1 was incubated at 37°C for 30 min before ARF6/GTP was added for 1 h at 37°C.