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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Mol Microbiol. 2014 Oct 16;94(4):898–912. doi: 10.1111/mmi.12807

Figure 3.

Figure 3

CTA1 activity in the absence or presence of ARF6.

For both panels, toxin activity against a diethylamino(benzylidine-amino)guanidine (DEA-BAG) substrate was detected from the increase in fluorescent units.

A. Two-fold dilutions of CTA1 were incubated at 25°C with ARF6/GTP (filled circles), ARF6/GDP (open circles), or in the absence of ARF6 (squares). Data are presented as the means ± standard errors of the means (SEMs) of 8 samples from two independent experiments.

B. Two-fold dilutions of CTA1 were incubated at 37°C in the presence of ARF6/GTP. Open squares represent toxin samples that were pre-incubated with ARF6/GTP at 25°C before warming to 37°C; filled squares represent toxin samples that were heated to 37°C for 30 min before the addition of ARF6/GTP. Data are presented as the means ± SEMs of 12 samples from three independent experiments.