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. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Mol Microbiol. 2014 Oct 16;94(4):898–912. doi: 10.1111/mmi.12807

Figure 5.

Figure 5

Raft-induced alterations to the activity of CTA1 in the absence or presence of ARF6.

A. Two-fold dilutions of CTA1 were incubated at 37°C with ARF6/GTP (squares), lipid raft LUVs (circles), or both lipid raft LUVs and ARF6/GTP (diamonds). Open symbols represent toxin samples that were pre-incubated with ARF6/GTP at 25°C before warming to 37°C; filled symbols represent toxin samples that were heated to 37°C for 30 min before the addition of LUVs or LUVs and ARF6/GTP. Toxin activity against a DEA-BAG substrate was detected from the increase in fluorescent units. Data are presented as the means ± SEMs of 8 samples from two independent experiments.

B. Two-fold dilutions of CTA1 were incubated at 37°C with ARF6/GTP (squares), non-raft LUVs (circles), or both non-raft LUVs and ARF6/GTP (diamonds). Open symbols represent toxin samples that were pre-incubated with ARF6/GTP at 25°C before warming to 37°C; filled symbols represent toxin samples that were heated to 37°C for 30 min before the addition of LUVs or LUVs and ARF6/GTP. Toxin activity against a DEA-BAG substrate was detected from the increase in fluorescent units. Four samples were used for each condition; data are presented as the means ± SEMs.