Skip to main content
. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Mol Microbiol. 2014 Oct 16;94(4):898–912. doi: 10.1111/mmi.12807

Figure 8.

Figure 8

Time- and concentration-dependent activation of CTA1 by ARF6/GTP.

A. 1 μg aliquots of CTA1 were incubated at 25°C in the presence of equimolar ARF6 and excess GTP. Toxin activity against the DEA-BAG substrate was detected from the increase in fluorescent units detected at the indicated time intervals. Four samples were used for each condition; data are presented as the means ± SEMs. One of two representative experiments is shown.

B. 1 μg aliquots of CTA1 were incubated at 25°C with the stated quantities of ARF6/GTP. Toxin activity against the DEA-BAG substrate was detected from the increase in fluorescent units after 2 h of incubation. Data are presented as the averages ± standard deviations from three replicate samples.