Skip to main content
. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Mol Microbiol. 2014 Oct 12;94(4):771–793. doi: 10.1111/mmi.12802

Figure 4. Dispersion in response to glutamate requires NicD DGC activity and the presence of the periplasmic 7TMR-DISMED2 domain.

Figure 4

Biofilms formed by P. aeruginosa PA14, ΔnicD, and ΔnicD mutant strains overexpressing nicD, nicD-GGAAF and nicDΔNoTMR under the control of the arabinose-inducible promoter of the pMJT1 vector were grown for 5 days in flow cells under flowing conditions. Multicopy expression of nicD but not nicD-GGAAF or nicDΔNoTMR restores biofilm dispersion upon glutamate exposure to wild type levels. Confocal images were acquired at the same position, prior to and following induction of dispersion by L-glutamate. Representative images are shown. White size bar, 100 µm. Blue vertical size bar (indicating height), 40 µm.