Fig 4. Induction of cadA in response to Zn(II) is increased in cells lacking BSH.
(A) Induction of PcadA-cat-lacZ as a function of Zn(II) concentration. Wild-type (HB11058) cells (diamonds) and an isogenic bshA null (HB11061) mutant (squares) carrying a cadA-cat-lacZ reporter fusion were grown to mid-logarithmic phase in LB medium with the indicated concentration of Zn(II) and assayed for β-galactosidase. (B) PcadA-lacZ activity in wild-type and mutant strains was monitored as a function of time after 50 µM Zn(II) addition. Representative results are shown for experiments repeated at least three times.