Figure 3.
Rescue of Adhesion Phenotypes by POUV Targets
(A) Rescue of ectoderm adhesion. Embryos were injected with 120 ng of Control-MO or PVD2 and 500 pg of mRNA encoding the indicated targets (1 ng for mOct4). Animal caps were cultured to neurula stage (stage 14). PVD2 animal caps displayed a dramatic loss of cell adhesion, which was rescued by murine Oct4 and by some of the conserved targets. Rescue index is reported as percentage of intact animal caps, and further details are summarized in Table S3.
(B) Rescue of adhesion phenotypes before neurulation. Animal cap adhesion assay is as described in (A) and photographed at late gastrula (stage 13) and neurula (stage 18) stages.
(C) Rescue of adherens junctions. Whole-mount immunofluorescence of embryos injected is as described in (A). Maximum intensity projections of confocal z stacks are shown.