Figure 4.

POUV Targets Rescue PVD2 Embryonic Phenotypes

(A) Rescue of blastopore closure. Photographs are of whole embryos at stage 12, injected as previously described.

(B) Quantification of blastopore rescue. Blastopore closure was estimated at stage 12 using the ratio of blastopore diameter to embryo diameter. The Xlim1 value was arbitrarily set to 1. Box plot shows maximum and minimum values, and the line represents the mean. p values were calculated by nonparametric ANOVA test with multiple comparison corrections (^∗p < 0.05, ^∗∗p < 0.001).

(C) Rescue of convergent extension defect in PVD2 morphants. In situ hybridization for the dorsal midline marker Xnot at stage 12 was used to evaluate convergent extension in PVD2 morphants and rescued embryos [40].

(D) Quantification of convergent extension based on the length/width ratio of Xnot (as shown by double arrows on Control-MO). Bars show mean ± SD. p values were calculated as described in (B). ^∗∗p < 0.001; ^∗∗∗p < 0.0001.

(E) Progenitor state gene expression was rescued by POUV targets. In situ hybridization for Xbra, vegetal view, and Bmp4, animal view, in PVD2 morphants is shown.

See also Figure S4.