Figure 1.

Expression of gal-7 in human mammary cancer tissues and cell lines. (A) Typical expression patterns of gal-7 in normal mammary tissues (myoepithelial cells) and in basal-like tumors collected from patients. Detailed immunohistochemical analysis in different types of breast cancer patients have been reported previously [6]. Scale bars, 300 μm (left) and 25 μm (right). (B) Cytosolic and nuclear fractions were purified from basal-like breast cancer cells MDA-MB-468. Expression of endogenous gal-7 was measured in both fractions by Western blotting. β-tubulin and lamin A/C are shown as positive cytoplasmic and nuclear expression controls, respectively. (C) Similar analysis showing expression of gal-7 in purified mitochondrial fractions. β-tubulin and COX IV are shown as positive cytoplasmic and mitochondrial expression controls, respectively. (D) Gal-7 in human breast cancer cell lines. Expression was measured by Western blot analysis from whole cell lysates. β-actin is shown as a positive expression control.