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. 2014 Nov 3;14:801. doi: 10.1186/1471-2407-14-801

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© Grosset et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Subcellular distribution of the R74S mutant. The effect of the R74S mutation on the (A) nuclear and (B) mitochondrial localization of gal-7, as measured by Western blot analysis of subcellular fractions. Membrane blots were incubated with anti-gal-7, anti-lamin A/C, anti-COX-IV and anti-β-tubulin. Lamin A/C, COX-IV and β-tubulin were used as nuclear, mitochondrial and cytosolic markers, respectively. (C) Whole MCF-7 cell and distribution of galectin-7 R74S mutant in (D) cytoplasm, (E) nucleus and (F) mitochondria, as measured by electron microscopy. Scale bars, 500 nm (C) and 100 nm (D-F).