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Acute activation of PVH neurons does not alter BAT UCP1 protein expression. BAT protein lysates were prepared from Sim1-Cre+3Dq and OXT-iCre+3Dq mice treated with either vehicle or CNO for 90 min. A, UCP1 protein levels are no different following Sim1PVH (left) or OXTPVH (right) activation with CNO compared with vehicle. B, Quantification of BAT UCP1 protein levels relative to the loading control, α-tubulin [Sim1+vehicle (veh), n = 2; Sim1+CNO, n = 2; OXT+veh, n = 3; OXT+CNO, n = 2].
