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Nos1PVH and OXTPVH neurons project to preganglionic neurons in the spinal cord. A–C, Sim1-Cre (A), Nos1-iCre (B), or OXT-iCre (C) mice with unilateral Ad-iN/syn-mCherry injections (red) show few projections to the RPa. D–F, In contrast, Sim1PVH (D), Nos1PVH (E), and OXTPVH (F) all innervate thoracic spinal cord regions in close proximity to cholinergic neurons of the intermediolateral column expressing ChAT (green; D′, E′, F′). Scale bars: A–C, 500 μm; D–F, 200 μm. cc, Central canal.
