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. 2014 Nov 12;34(46):15306–15318. doi: 10.1523/JNEUROSCI.0226-14.2014

Figure 5.

Figure 5.

DREADDs allow for remote and temporal control of PVH neuronal activity. A, Diagram of the Cre-dependent hM3Dq DREADDs expression vector. B–D, Expression of AAV-hM3Dq-mCherry in the PVH of Sim1-Cre (B), Nos1-iCre (C), or OXT-iCre (D) mice is detected by IHC. E–G, Sim1-Cre (E), Nos1-iCre (F), or OXT-iCre (G) mice with bilateral AAV-hM3Dq injections demonstrate little nuclear Fos immunoreactivity after treatment with vehicle. H–J, In contrast, PVH neurons expressing hM3Dq are activated following injection of CNO. OXTPVH neuronal activation leads to nuclear Fos expression (J, inset, green) not only in hM3Dq-expressing OXTPVH neurons (J, inset, red, closed arrowheads), but also in neighboring non-OXTPVH neurons (open arrowheads). Scale bars: B–D, 1 mm; E–J, 200 μm. oc, Optic chiasm.